ESTONIAN ACADEMY
PUBLISHERS
eesti teaduste
akadeemia kirjastus
PUBLISHED
SINCE 1952
 
Proceeding cover
proceedings
of the estonian academy of sciences
ISSN 1736-7530 (Electronic)
ISSN 1736-6046 (Print)
Impact Factor (2020): 1.045

Kinetic tools for the identification of ligand–receptor interaction mechanisms; pp 202–213

Full article in PDF format | https://doi.org/10.3176/proc.2017.2.08

Authors
Siim Kukk, Peep Miidla, Jaak Järv

Abstract

Tools for the identification of receptor–ligand interaction mechanisms were developed by mathematical modeling of the influence of a ligand on the kinetics of a reporter ligand binding with a receptor. This approach allows kinetic differentiation between ligands of both rapid and slow binding modes, but also distinguishes compounds that share the same binding site with the reporter ligand or bind non-competitively to a distinct binding site. In order to simulate the kinetic behavior of this system, a mathematical model comprising ordinary differential equations was derived and solved numerically.


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