, Reelika Rätsep , Marge Malbe, Hedi Kaldmäe , Sirje Kuusik , Piret Raudsepp , Asta-Virve Libek , Ave Kikas Twenty-two blackcurrant genotypes, ‘Karri’, ‘Almo’, ‘Ats’, ‘Elo’, ‘Varmas’, ‘Mairi’, ‘Asker’, ‘Pilėnai’, ‘Vyčiai’, ‘Ben Alder’, ‘Ben Nevis’, ‘Ben Lomond’, ‘Ben Sarek’, ‘Intercontinental’, ‘Titania’, ‘Öjebyn’, ‘Ļentjai’, ‘Pamyati Vavilova’, ‘Zagadka’, 10B, 1-96-16, and 2-96-51, were evaluated in two subsequent years. The aim of the research was to compare berry weight, seed content in the berries, and fatty acid profile in the seeds. The composition of fatty acids was identified and quantified using gas chromatography.
Blackcurrant berry weight varied between 1.0 and 1.7 g, depending on the genotype, being the highest in genotypes ‘Mairi’, ‘Karri’, ‘Intercontinental’, and ‘Ļentjai’. Seed content in berries ranged from 2.2% to 4.6% and was the highest in ‘Karri’ and ‘Elo’. Oil content correlated positively with the proportion of seeds per fresh weight of berries. Therefore, the highest seed content correlated with the highest oil content per fresh weight of berries in ‘Karri’ and ‘Elo’, 1.2% and 1.3%, respectively. On average over two years, the total oil content in the seeds ranged from 24.4% to 31.2%, showing the highest levels in ‘Ben Nevis’, ‘Pamyati Vavilova’, and ‘Asker’. The most abundant fatty acid identified in blackcurrant seeds was linoleic acid, ranging from 40.1% to 48.6%, followed by α-linolenic acid, from 11.7% to 16.5%, oleic acid, from 10.3% to 16.4%, and γ-linolenic acid, from 10.9% to 15.6%.
Basegmez, H. I. O., Povilaitis, D., Kitrytė, V., Kraujalienė, V., Šulniūtė, V., Alasalvar, C. et al. 2017. Biorefining of blackcurrant pomace into high value functional ingredients using supercritical CO2, pressurized liquid and enzyme assisted extractions. The Journal of Supercritical Fluids, 124, 10–19.
https://doi.org/10.1016/j.supflu.2017.01.003
Beattie, J., Crozier, A. and Duthie, G. G. 2005. Potential health benefits of berries. Current Nutrition & Food Science, 1(1), 71–86.
https://doi.org/10.2174/1573401052953294
Chauhan, A. S., Patel, A. K., Nimker, V., Singhania, R. R., Chen, C.-W., Patel, A. K. et al. 2024. Biorefining of essential polyunsaturated fatty acids from microbial sources: current updates and prospects. Systems Microbiology and Biomanufacturing, 4, 425‒447.
https://doi.org/10.1007/s43393-023-00207-x
Dekeyser, K. and Rampa, F. 2023. Upgrading the EU’s policy toolbox for nutrition leadership. https://ecdpm.org/work/upgrading-eus-policy-toolbox-nutrition-leadership (accessed 2025-02-19).
Flores, G. and Ruiz del Castillo, M. L. 2016. Enhancement of nutritionally significant constituents of black currant seeds by chemical elicitor application. Food Chemistry, 194, 1260–1265.
https://doi.org/10.1016/j.foodchem.2015.09.006
Goffman, F. D. and Galletti, S. 2001. Gamma-linolenic acid and tocopherol contents in the seed oil of 47 accessions from several Ribes species. Journal of Agricultural and Food Chemistry, 49(1), 349–354.
https://doi.org/10.1021/jf0006729
Gustinelli, G., Eliasson, L., Svelander, C., Andlid, T., Lundin, L., Ahrné, L. et al. 2018. Supercritical fluid extraction of berry seeds: chemical composition and antioxidant activity. Journal of Food Quality, 2018(1), 046074.
https://doi.org/10.1155/2018/6046074
Hendawy, O., Gomaa, H. A. M., Hussein, S., Alzarea, S. I., Qasim, S., Abdel Rahman, F. E.-Z. S. et al. 2021. Cold-pressed raspberry seeds oil ameliorates high-fat diet triggered non-alcoholic fatty liver disease. Saudi Pharmaceutical Journal, 29(11), 1303–1313.
https://doi.org/10.1016/j.jsps.2021.09.014
Jurgoński, A., Koza, J., Chu, D.-T. and Opyd, P. M. 2018. Berry seed oils as potential cardioprotective food supplements. Nutrire, 43, 26.
https://doi.org/10.1186/s41110-018-0086-x
Kahu, K., Jänes, H., Luik, A. and Klaas, L. 2009. Yield and fruit quality of organically cultivated blackcurrant cultivars. Acta Agriculturae Scandinavica Section B ‒ Soil & Plant Science, 59(1), 63–69.
https://doi.org/10.1080/09064710701865139
Kaldmäe, H., Kikas, A., Arus, L. and Libek, A.-V. 2013. Genotype and microclimate conditions influence ripening pattern and quality of blackcurrant (Ribes nigrum L.) fruit. Zemdirbyste-Agriculture, 100(2), 167–174.
https://doi.org/10.13080/z-a.2013. 100.021
Kikas, A., Kahu, K., Arus, L., Kaldmäe, H., Rätsep, R. and Libek, A.-V. 2017. Qualitative properties of the fruits of blackcurrant Ribes nigrum L. genotypes in conventional and organic cultivation. Proceedings of the Latvian Academy of Sciences, Section B. Natural, Exact, and Applied Sciences, 71(3), 190–197.
https://doi.org/10.1515/prolas-2017-0032
Konopka, I., Tańska, M., Dąbrowski, G., Ogrodowska, D. and Czaplicki, S. 2023. Edible oils from selected unconventional sources ‒ a comprehensive review of fatty acid composition and phytochemicals content. Applied Sciences, 13(23), 12829.
https://doi.org/10.3390/app132312829
Kuhnt, K., Degen, C., Jaudszus, A. and Jahreis, G. 2012. Searching for health beneficial n-3 and n-6 fatty acids in plant seeds. European Journal of Lipid Science and Technology, 114(2), 153–160.
https://doi.org/10.1002/ejlt.201100008
Libek, A. and Kikas, A. 2002. Evaluation of blackcurrant cultivars in Estonia. Acta Horticulturae, 585, 209‒213.
https://doi.org/10.17660/ActaHortic.2002.585.33
Michalak, M. and Kiełtyka-Dadasiewicz, A. 2018. Oils from fruit seeds and their dietetic and cosmetic significance. Herba Polonica, 64(4), 63–70.
https://doi.org/10.2478/hepo-2018-0026
Omachi, D. O., Aryee, A. N. A. and Onuh, J. O. 2024. Functional lipids and cardiovascular disease reduction: a concise review. Nutrients, 16(15), 2453.
https://doi.org/10.3390/nu16152453
Pieszka, M., Tombarkiewicz, B., Roman, A., Migdal, W. and Niedziolka, J. 2013. Effect of bioactive substances found in rapeseed, raspberry and strawberry seed oils on blood lipid profile and selected parameters of oxidative status in rats. Environmental Toxicology and Pharmacology, 36(3), 1055–1062.
https://doi.org/10.1016/j.etap.2013.09.007
Pieszka, M., Migdał, W., Gąsior, R., Rudzińska, M., Bederska-Łojewska, D., Pieszka, M. et al. 2015a. Native oils from apple, blackcurrant, raspberry, and strawberry seeds as a source of polyenoic fatty acids, tocochromanols, and phytosterols: a health implication. Journal of Chemistry, 2015, 59541.
https://doi.org/10.1155/2015/659541
Pieszka, M., Gogol, P., Pietras, M. and Pieszka, M. 2015b. Valuable components of dried pomaces of chokeberry, black currant, strawberry, apple and carrot as a source of natural antioxidants and nutraceuticals in the animal diet. Annals of Animal Science, 15(2), 475–491.
https://doi.org/10.2478/aoas-2014-0072
Radočaj, O., Vujasinovic, V., Dimic, E. and Basic, Z. 2014. Blackberry (Rubus fruticosus L.) and raspberry (Rubus idaeus L.) seed oils extracted from dried press pomace after longterm frozen storage of berries can be used as functional food ingredients. European Journal of Lipid Science and Technology, 116(8), 1015–1024.
https://doi.org/10.1002/ejlt.201400014
Rosell, M. and Fadnes, L. T. 2024. Vegetables, fruits, and berries – a scoping review for Nordic Nutrition Recommendations 2023. Food & Nutrition Research, 68.
https://doi.org/10.29219/fnr.v68.10455
Ruiz del Castillo, M. L. and Dobson, G. 2002. Varietal differences in terpene composition of blackcurrant (Ribes nigrum L) berries by solid phase microextraction/gas chromatography. Journal of the Science of Food and Agriculture, 82(13), 1510–1515.
https://doi.org/10.1002/jsfa.1210
Ruiz del Castillo, M. L., Dobson, G., Brennan, R. and Gordon, S. 2002. Genotypic variation in fatty acid content of blackcurrant seeds. Journal of Agricultural and Food Chemistry, 50(2), 332–335.
https://doi.org/10.1021/jf010899j
Sarv, V., Hussain, S., Rätsep, R. and Kikas, A. 2024. The proximate composition, mineral and pectin content and fatty acid profile of the pomace fraction of 16 rowanberry cultivars. Plants, 13(12), 1615.
https://doi.org/10.3390/plants13121615
Šavikin, K. P., Dordevic, B. S., Ristic, M. S., Krivokuca-Dokic, D., Pljevljakušic, D. S. and Vulic, T. 2013. Variation in the fatty-acid content in seeds of various black, red, and white currant varieties. Chemistry and Biodiversity, 10(1), 157–165.
https://doi.org/10.1002/cbdv.201200223
Sheppe, A. E. F. and Edelmann, M. J. 2021. Roles of eicosanoids in regulating inflammation and neutrophil migration as an innate host response to bacterial infections. Infection and Immunity, 89.
https://doi.org/10.1128/iai.00095-21
Sibson, R. 1973. SLINK: an optimally efficient algorithm for the single-link cluster method. The Computer Journal, 16(1), 30–34.
https://doi.org/10.1093/comjnl/16.1.30
Sławińska, N., Prochoń, K. and Olas, B. 2023. A review on berry seeds ‒ a special emphasis on their chemical content and health-promoting properties. Nutrients, 15(6), 1422.
https://doi.org/10.3390/nu15061422
Sukhija, P. S. and Palmquist, D. L. 1988. Rapid method for determination of total fatty acid content and composition of feedstuffs and feces. Journal of Agricultural and Food Chemistry, 36(6), 1202–1206.
https://doi.org/10.1021/jf00084a019
Tabart, J., Kevers, C., Pincemail, J., Defraigne, J.-O. and Dommes, J. 2006. Antioxidant capacity of black currant varies with organ, season, and cultivar. Journal of Agricultural and Food Chemistry, 54(17), 6271–6276.
https://doi.org/10.1021/jf061112y
Tervise Arengu Instituut. 2025. Eesti riiklikud toitumise, liikumise ja uneaja soovitused. Tabelraamat (Estonian national recommendations for nutrition, exercise, and sleep. Table book).
https://www.tai.ee/et/valjaanded/eesti-riiklikud-toitumise-liikumise-ja-uneaja-soovitused-tabelraamat (accessed 2025-02-14).
Wójciak, M., Mazurek, B., Tyśkiewicz, K., Kondracka, M., Wójcicka, G., Blicharski, T. et al. 2022. Blackcurrant (Ribes nigrum L.) seeds ‒ a valuable byproduct for further processing. Molecules, 27(24), 8679.
https://doi.org/10.3390/molecules27248679
Yang, B., Ahotupa, M., Määttä, P. and Kallio, H. 2011. Composition and antioxidative activities of supercritical CO2-extracted oils from seeds and soft parts of northern berries. Food Research International, 44(7), 2009–2017.
https://doi.org/10.1016/j.foodres.2011.02.025
