Advances in web-based education and technology-mediated learning environments offer opportunities to enhance knowledge access and active learning, particularly in infection control, a critical public health issue. This study aims to assess the quality and reliability of e-learning materials used in infection control education for nursing students by evaluating materials related to hand hygiene, personal protective equipment, isolation measures, and standard precautions in healthcare colleges across Estonia, Finland, Portugal, Spain, and Turkey. The materials were assessed by ten experts from each country (only six experts from Finland) via the Learning Object Review Instrument (LORI). In our study, the Cronbach’s alpha coefficient for e-learning materials ranged from 0.736 to 0.932. In terms of the mean LORI total score, the highest mean score was for personal protective equipment (3.983 ± 0.619), whereas the lowest was for standard precautions (3.850 ± 0.550). When comparing the quality of materials related to infection control measures among the countries, materials related to hand hygiene, personal protective equipment, isolation measures, and standard precautions from Spain had the highest LORI total scores. In contrast, materials from Portugal had the lowest mean scores (with the exception of isolation measures). The interobserver agreement coefficients were calculated as 0.516–0.805 for Spain, 0.614–0.729 for Estonia, 0.511–0.711 for Turkey, 0.749–0.938 for Finland, and 0.566–0.882 for Portugal. As the first study to evaluate the quality and reliability of infection control materials via LORIs, the findings contribute valuable insights to the literature and provide a foundation for future research on improving e-learning in nursing education.
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