This study evaluates applicability of microbial sensors based on Pseudomonas putida P67.2 (Ps. p.) and Pseudomonas fluorescens P75 (Ps. fl) for BOD measurements in the phenol-containing wastewaters that mimic the wastewaters from oil shale industry. Sensors are calibrated with OECD synthetic wastewater. Linear range in calibration solution for Ps. p. and Ps. fl. sensors is BOD 0-50 mg/L and 0-65 mg/L, respectively. The steady state response time for both sensors is 15-45 min. Measurements show that although Ps. fl. has better sensitivity to calibration solution, the concurrence between sensor-BOD and BOD7 in phenol-spiked wastewater is better for Ps. p. sensor. In linear range the error is only 1.2–13%. For Ps. fl. the error in linear range is 17–55%. Preconditioning in phenol solution (BOD = 5 mg/L) increases sensor’s sensitivity to phenol considerably (52%). This study demonstrates that preconditioned microbial sensors with specifically selected cultures can give better results for fast BOD determination in specific wastewaters.
1. Handique, J. G., Baruah, J. B. Polyphenolic compounds: an overview // React. Funct. Polym. 2002. Vol. 52, No. 3. P. 163–188.
doi:10.1016/S1381-5148(02)00091-3
2. Amlathe, S., Upadhyay, S., Gupta, K. V. Spectrophotometric determination of trace amounts of phenol in waste water and biological fluids // Analyst. 1987. Vol. 112, No. 10. P. 1463–1465.
3. Rätsep, A., Toomik, A. // Hydrotechnogenic influxes into the Purtse basin rivers in North-East Estonia / Energy, Environment and Natural Resoures Management in the Baltic Sea Region – 4th International Conference on System Analysis. Nordiske Seminar og Arbejdsrapporter, 1993. Vol. 653. P. 459–462.
4. Timur S., Pazarlioglu, N., Pilloton, R., Telefoncu, A. Detection of phenolic compounds by thick film sensors based on Pseudomonas putida // Anal. Chim. Acta. 2003. Vol. 61, No. 2. P. 87–93.
5. APHA. Standard Methods for Examination of Water and Wastewater. – Washington, DC: American Public Health Association, American Water Works Association, American Water Environment Federation. Washington, 1992.
6. D’Souza, S. F. Microbial biosensors review // Biosens. Bioelectron. 2001. Vol. 16, No. 6. P. 337–353.
doi:10.1016/S0956-5663(01)00125-7
7. Riedel, K., Renneberg, R., Wollenberg, U., Kaiser, G., Scheller, W. Microbial sensors. Fundamentals and application for process control // J. Chem. Technol. Biotechnol.1989. Vol. 44. P. 85–106.
8. Organization for Economic Cooperation and Development (OECD), 1986. Standard Methods for the Examination of Waters and Wastewater. American Public Health Association, Washington, DC, 16th ed. P. 525–531.
9. Miller, J. H. A short course in bacterial genetics. – New York, 1972.
10. Kim, M.-N., Park, K.-H. Klebsiella BOD sensor // Sens. Actuators. 2001. Vol. 4030, P. 1–6.
11. Liu, J., Mattiasson, B. Microbial BOD sensors for wastewater analysis // Water Res. 2002. Vol. 36, No. 15. P. 3786–3802.
doi:10.1016/S0043-1354(02)00101-X
12. Liu, J., Björnsson, L., Mattiasson, B. Immobilised activated sludge based biosensor for biochemical oxygen demand measurment // Biosens. Bioelectron. 2000. Vol. 14, No. 12. P. 883–893.
doi:10.1016/S0956-5663(99)00064-0
13. Eggins, B. Biosensors: An Introduction. – Wiley-Teubner, 1996. P. 1–10, 31–35, 133–135.
14. Li, F., Tan, T. C., Lee, Y. K. Effects of pre-conditioning and microbial composition on the sensing efficacy of a BOD biosensor // Biosens. Bioelectron. 1994. Vol. 9, No. 3. P. 197–205.
doi:10.1016/0956-5663(94)80122-3
doi:10.1016/S0956-5663(01)00115-4
