New stable carbon and nitrogen isotopic analyses from medieval and early modern sites across Estonia demonstrate systematic differences in the dietary habits of people from various locations and social groups. These results are compared with previously published isotopic data from similar contexts to identify the type and origin of dietary items, specifically in terms of aquatic resource consumption. Distinction between protein sources is, however, complicated by the high degree of isotopic variation among aquatic ecosystems and the fact that resources from multiple habitats were routinely exploited, resulting in a mixing of the isotopic signal. Nitrogen isotopic ratios display variations in the consumption of higher trophic level protein (such as fish), differentiating between rural, urban and elite individuals, as well as between males and females. Carbon isotopic ratios show a clear distinction between humans from coastal and inland sites, likely reflecting the importance of Baltic Sea fish to coastal communities. However, the exact quantification of aquatic resources into the diets of historic period people in the region needs further work.
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